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- Feb 19, 2007
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Ok, here's an interesting dilemma we've been having in our lab lately, and none of us can come up with a valid reason behind it, so for those of you that have any ideas as to what's going wrong, PLEASE help us out here!
This is how the PCR used to look when all things were running correctly (the lower bands are Internal Controls, the higher bands are positive reactions)
And this is how our PCR has been looking for the last few weeks :
So this has been quite baffling for us. We have tried new primers, polymerases, templates, and buffers, but keep getting this same result. If anybody has any ideas or solutions, we're all ears! Thanks for your help, and for making SDN rock!
This is how the PCR used to look when all things were running correctly (the lower bands are Internal Controls, the higher bands are positive reactions)
And this is how our PCR has been looking for the last few weeks :
So this has been quite baffling for us. We have tried new primers, polymerases, templates, and buffers, but keep getting this same result. If anybody has any ideas or solutions, we're all ears! Thanks for your help, and for making SDN rock!