Hello, just a quick question about protein concentration quantification.
I followed this procedure:
https://www.piercenet.com/instructions/2160129.pdf
with the test tube procedure.
I used the chart on page 2 to dilute the BSA standard and then graphed the absorbance and got a polynomial equation. Then I took a mL of my unknown sample and followed the same exact chart for the dilutions and then found the absorbance of each dilution.
Was I supposed to only take 30 uL of each of my unknown samples and mix it with the dye instead of doing the serial dilution? Otherwise, I would only know the concentration of 1 sample right?
Furthermore, how do I convert the polynomial equation to solve in terms of X (concentration) so I can simply plug in the absorbance values (Y) of my unknown to find the concentration.
Thanks!
