Blotting Techniques

[kryptonxenon]

Which technique CANNOT be used to analyze gene expression?
A. Western blotting
B. Northern blotting
C. Southern blotting
D. rtPCR

Since gene expression implies formation of a protein I was thinking that B, C, and D could be right. Why is C correct?

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[theonlytycrane]

C can't be used because southern just detects for a DNA sequence. I think you're correct, the question stem is just asking for the one that wouldn't work.