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- Thread starter fa21212
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Hi @fa21212 -
Thanks for the question! It sounds like the underlying point of confusion is that membrane-bound enzymes -- even those facing the cytosol -- will not be found together with cytosolic enzymes after centrifugation. "Cytosolic enzymes" are those that are freely floating around in the aqueous environment, whereas membrane-bound enzymes will be found in the membrane fraction. Once you eliminate choice D, the Q becomes about which location is most likely given the role of thiokinase in converting FAs into their CoA derivatives.
Hope this is helpful!
Thanks for the question! It sounds like the underlying point of confusion is that membrane-bound enzymes -- even those facing the cytosol -- will not be found together with cytosolic enzymes after centrifugation. "Cytosolic enzymes" are those that are freely floating around in the aqueous environment, whereas membrane-bound enzymes will be found in the membrane fraction. Once you eliminate choice D, the Q becomes about which location is most likely given the role of thiokinase in converting FAs into their CoA derivatives.
Hope this is helpful!