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- Oct 21, 2015
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Hi I am having trouble with these following question
A description of the structures of four proteins is shown bellow
Protein Description
Protein 1 32 kDa monomer
Protein 2 Disulfide-linked homodimer comprised of 19 kDa monomers
Protein 3 Homotrimer comprised of 25 kDa monomers
Protein 4 Homodimer comprised of 38 kDa monomers
Which protein has the highest electrophoretic mobility in SDS-PAGE under non-reducing conditions? (Note: There are no disulfide interactions unless stated in the table.)
So the answer is protein 3 because it's 25KDA total which is the lowest compared to other three proteins. It's question 64 in section bank for chem if you have access. My question is how the total mass of protein 3 won't be 25*4 = 100 since it's homotrimer which i thought means four monomers.
Thanks.
A description of the structures of four proteins is shown bellow
Protein Description
Protein 1 32 kDa monomer
Protein 2 Disulfide-linked homodimer comprised of 19 kDa monomers
Protein 3 Homotrimer comprised of 25 kDa monomers
Protein 4 Homodimer comprised of 38 kDa monomers
Which protein has the highest electrophoretic mobility in SDS-PAGE under non-reducing conditions? (Note: There are no disulfide interactions unless stated in the table.)
So the answer is protein 3 because it's 25KDA total which is the lowest compared to other three proteins. It's question 64 in section bank for chem if you have access. My question is how the total mass of protein 3 won't be 25*4 = 100 since it's homotrimer which i thought means four monomers.
Thanks.